guaD [2019-08-15 10:23:57]

guanine deaminase, general stress protein, important for survival of ethanol and paraquat stresses

Locus: BSU_13170

Isoelectric point: 5.00

Molecular weight: 17.01 kDa

Protein length: 156 aa

Gene length: 471 bp

Function: deamination of guanine to xanthine, purine salvage and interconversion

Product: guanine deaminase

Essential: no

E.C.: 3.5.4.3

Synonyms: gde,yknA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Purine salvage and interconversion
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 1,382,677 → 1,383,147

The protein

Catalyzed reaction/ biological activity

• guanine + H⁺ + H₂O --> NH₄⁺ + xanthine (according to UniProt)

Protein family

• Cytidine and deoxycytidylate deaminase family (according to UniProt)

Domains

• CMP/dCMP-type deaminase domain (aa 2-120) (according to UniProt)

Structure

• 1WKQ

Expression and Regulation

Operons

• Genes: guaD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • PucR: activation, PubMed, in PucR regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-A752 (yknA::erm), available at the NBRP B. subtilis, Japan
• BKE13170 (ΔguaD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA, downstream forward: _UP4_TAAAAGGATCAGGCATGCGC
• BKK13170 (ΔguaD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA, downstream forward: _UP4_TAAAAGGATCAGGCATGCGC

References

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